Offres d’emploi

Cet espace est destiné à recevoir des offres émanant de laboratoires (emploi, post-doc, recherche de candidats pour thèse) comme des demandes émanant d’étudiants (recherche de laboratoires pour un recrutement, recherche de stages).

Ces offres et/ou demandes sont à envoyer au secrétaire de l’association : hubert.schaller@ibmp-cnrs.unistra.fr

21 avril 2020 PhD

Study of the mechanisms of lipids oxidation and antioxidant in complex food matrices : Example of infant formulas enriched in polyunsaturated fatty acids

PhD Offer

Contract Type : 3 years Doctoral contract CIRAD/INRAE
Starting date : Last trimester 2020

Pierre Villeneuve
ATTENTION NOUVELLE ADRESSE ET NOUVEAU NUMERO DE TEL

CIRAD/UMR IATE
TA B-62 / 16
Maison de la Technologie / Bât 16
73 rue Jean-François Breton
34398 MONTPELLIER Cedex 05

Tel/Phone (33) (0)4 67 61 49 84
http://umr-iate.cirad.fr/

Study of the mechanisms of lipids oxidation and antioxidant in complex food matrices : Example of infant formulas enriched in polyunsaturated fatty acids

PhD Summary : Food products displaying high content in polyunsaturated fatty acids are crucial for infant nutrition but particularly susceptible to lipid oxidation. Such oxidative alteration leads to the degradation of the food product nutritionnal profile and to the potential formation of toxic compounds. In food matrices, lipids are most of the time found under dispersed form (it is also the case in powders) and their oxidative behaviour is very complex. Indeed, in such heterogeneous systems, oxidation occurs primarily at oil-water interface where pro-oxidant agents come into contact with oxidable substrate (lipid) and initiate the reaction. In this context, our previous studies on model emulsions (oil-in-water) have demonstrated the importance of using antioxidant molecules able to locate predominantly at interface to optimize their activity. Similarly, we have demonstrated the key role of other tensioactive substances (emulsifier, proteins…) which can act as traps for pro- or antioxidants in the system and modulate the kinetics of oxidation. However up to now the transport and diffusion of radicals within heterogeneous food matrices is not described. And yet, we are convinced that a better understanding of these dynamics of transfer between the various components of the food systems (micellar phase-interface-lipid susbstrate) would lead to a better stabilization of food systems enriched in polyunsaturated fatty acids. The objective of this thesis will be to elucidate the mechanisms of propagation of lipid oxidation whithin heterogeneous food products, taking unsaturated fatty acids enriched infant formulas as an example. We will first work on model emulsion systems mimicking these products to unveil the mechanims of diffusion of oxidation using confocal imaging with fluorescent probes reporter of oxidation or RAMAN imaging. Then we will study the influence of natural antioxidants or proteins, used either isolated, or associated under the form of natural assemblies (vesicles, membrane fragments…) on the modulation of oxidation kinetics and will model these kinetics. In a second part of the thesis, we will validate these results in a real set of food product: infant formula in powder form. We aim at demonstrating that formulation and potentially process modification of this food product can improve its resistance to oxidation and thus improve its nutritionnal properties during storage.

Lipid Composition and State of Oxidation of Fortified Infant Flours in Low-Income Countries Are Not Optimal and Strongly Affected by the Time of Storage. Moustiés C, Bourlieu C, Baréa B, Servent A, Alter P, Lebrun M, Hemery Y, Laillou A, Avallone S. Eur. J. Lipid Sci. Technol 2019. https://doi.org/10.1002/ejlt.201900173
The need for a new step in the study of lipids oxidation in heterophasic systems. Villeneuve P, Durand E, Decker EA. J. Agric. Food Chem. 2018. https://doi.org/10.1021/acs.jafc.8b03603
Eleostearic Phospholipids as Probes to Evaluate Antioxidants Efficiency against Vesicles Liposomes Oxidation. Durand E, Delavault A, Bourlieu C, Lecomte J, Figueroa M, Decker EA, Michel-Salaun F, Kergourlay G, Villeneuve P. Chem. Phys. Lipids. 2017. https://doi.org/10.1016/j.chemphyslip.2017.10.006
A Comparison of Antioxidant Evaluation Assays for Investigating Antioxidative Activity of Gallic Acid and Its Alkyl Esters in Different Food Matrices. Phonsatta N, Deetae P, Luangpituksa P, Grajeda Iglesias C, Figueroa-Espinoza MC, Lecomte J, Villeneuve P, Decker EA, Visessanguan W, Panya A. J. Agric. Food Chem. 2017. https://doi.org/10.1021/acs.jafc.7b02503
Hurdles in Predicting Antioxidant Efficacy in Oil-in-Water Emulsions. Decker EA, Mc Clements DJ, Bourlieu-Lacanal C, Durand E, Figueroa-Espinoza MC, Lecomte J, Villeneuve P. Trends Food Sci. Technol. 2017. https://doi.org/10.1016/j.tifs.2017.07.001
Supervisors :
Claire BOURLIEU (UMR IATE) et Pierre VILLENEUVE (UMR QUALISUD)
Doctoral School : GAIA (N°584), Montpellier, France
Hosting Teams : UMR QUALISUD/UMR IATE
The PhD candidate will work in two Joint Research Units (UMR Qualisud and UMR IATE), both located in Montpellier, France.

General Requirements
Applicants should have a background in Chemistry, Food Chemistry, Nutrition. Good Knowledge of english. Highly motivated by research, autonomous, dynamic.

Candidacy must be sent to C. Bourlieu (claire.bourlieu-lacanal@inra.fr) and P. Villeneuve (villeneuve@cirad.fr) and should contain :
– Curriculum
– Personal statement
– Letters of recommendation (2)
– Master grades

Contract Type : 3 years Doctoral contract CIRAD/INRAE
Starting date : Last trimester 2020

Pierre Villeneuve
ATTENTION NOUVELLE ADRESSE ET NOUVEAU NUMERO DE TEL

CIRAD/UMR IATE
TA B-62 / 16
Maison de la Technologie / Bât 16
73 rue Jean-François Breton
34398 MONTPELLIER Cedex 05

Tel/Phone (33) (0)4 67 61 49 84
http://umr-iate.cirad.fr/

20 avril 2020

The Bioscience and biotechnology Institute of Aix-Marseille (BIAM) is looking to recruit experienced and emerging scientific talents!

 

The Bioscience and biotechnology Institute of Aix-Marseille (BIAM) is looking to recruit experienced and emerging scientific talents!
Situated in the south of France in the Provence-Alpes-Côte d’Azur, the BIAM is a joint research center between the Commissariat à l’énergie atomique et aux énergies alternatives (CEA), the Centre National de la Recherche Scientifique (CNRS) and Aix-Marseille Université.

The BIAM proposes an ambitious strategic and scientific project “Green solutions for tomorrow” that concentrates excellent fundamental, technological research and innovation by bringing together leading research teams and economic partners.

The project is organised around 2 fundamental research themes “energy bioconversion” and “the response and adaptation of living organisms to environmental constraints”

Apply to the Call for International Mobility

 

As of the summer 2020, experienced scientists and talented early career researchers are invited to join BIAM to strengthen the Institute’s scientific project, either through mobility or appointment. Two permanent high-level researcher positions (PhD + a minimum of 2 years experience) are to be filled on the Cadarache site as part of the “Green solutions for tomorrow” project to reinforce existing teams or to promote transversality between exisiting themes.
SEE ALSO

Video about microalgae activities in the “Cité des Energies”

Adresse

CEA Cadarache
DRF-BIAM Bât 177
13108 Saint-Paul-Lez-Durance

 

 

 

 

 
 

 

 

 

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31 octobre 2020 CDD 18 mois (possible 24 mois selon conditions) au laboratoire Physiologie Cellulaire et Végétale, CEA Grenoble

Nous recherchons : - un/e Technicien/ne en ingénierie génomique et lipidomique des diatomées

 

La mission s’intègre dans un projet de recherche visant à approfondir nos connaissance du métabolisme lipidique chez une diatomée marine .

 Contact : envoyer CV et lettre de motivation à Alberto Amato (alberto.amato@cea.fr) et Eric Maréchal (eric.marechal@cea.fr) AVANT LE 15 FEVRIER 2020

 Laboratoire de Physiologie Cellulaire & Végétale

UMR 5168 CNRS-CEA-INRA-Univ. Grenoble Alpes / INRA UMR 1417

IRIG, CEA-Grenoble

17 rue des Martyrs, 38000 Grenoble, France

 http://www.lpcv.fr/en

+33 (0)4 38 78 49 85

 

Description :

 

Technicien/ne en ingénierie génomique et lipidomique des diatomées.

Le Laboratoire de Physiologie Cellulaire et Végétale (LPCV ; Institut de Recherche Interdisciplinaire de Grenoble – IRIG –CEA) recherche un/e candidat/e pour un poste de technicien dans l’équipe Lipides pour travailler sur un groupe spécifique d’algues appelées diatomées. Le/la technicien/ne contribuera en particulier à la caractérisation de gènes impliqués dans la synthèse des lipides de Phaeodactylum tricornutum. Les diatomées sont des organismes photosynthétiques unicellulaires et P. tricornutum est considérée comme un organisme modèle pour l’étude de leur biologie et physiologie, ainsi que pour le développement d’approches biotechnologiques. De nombreux outils moléculaires sont disponibles pour P. tricornutum, notamment pour la transformation génétique et l’ingénierie génomique tel que les technologies TALEN et CRISPR/Cas9. Des « knock out » et « knock down » de gènes seront réalisés afin de produire des mutants exprimant partiellement ou n’exprimant plus un ou plusieurs gènes d’intérêt. Une analyse du phénotype lipidique permettra de caractériser les fonctions des enzymes codées par ces gènes.

Le/la technicien/ne partricipera à la construction de vecteurs d’expression pour l’ingénierie génomique et la transformation de P. tricornutum par des méthodes de biolistique. Le/la technicien/ne sera également en charge du criblage génétique et phénotypique de ces souches mutantes via une méthode de criblage récemment développée au sein du laboratoire. Le contenu en lipides des souches les plus prometteuses sera analysé par GC-MS et LC-MS/MS afin de caractériser les fonctions des gènes d’intérêt.

Une partie du temps sera aussi dédiée à un projet en collaboration avec une entreprise privée, qui s’intéresse à l’amélioration de la production de lipides dans différents protistes marins.  

 

Diplôme requis :

  • Licence professionnelle biotechnologies ou Biologie option microbiologie ou biologie végétale ou culture cellulaire ou biochimie

Compétences requises :

  • Manipulation de données et mise en forme des résultats
  • Clonage
  • Culture d’algue ou d’autres micriorganismes en conditions stériles
  • Aisance en communication écrite et orale
  • Travail de manière autonome et en groupe
  • Implication dans plusieurs projets en parallèle
  • Adaptation à de nouveaux sujets, esprit critique et créatif afin de s’adapter aux difficultés

 

Durée 18 mois à 24 mois.

Lieu : LPCV Grenoble (http://www.lpcv.fr/)

Envoyer CV + lettre de motivation à Alberto Amato (alberto.amato@cea.fr) et Eric Maréchal (eric.marechal@cea.fr) avant le 15 Février 2020

 

Technician in genomic engineering and lipidomics of a marine diatom

The Laboratory of Cell and Plant Physiology (Laboratoire de Physiologie Cellulaire et Végétale; Interdisciplinary Research Institute in Grenoble – IRIG – CEA) opens a position in the Lipid team to work on a specific group of algae called the diatoms. The selected technician will contribute the characterisation of genes involved in the synthesis of lipids in the marine diatom Phaeodactylum tricornutum. Diatoms are unicellular photosynthetic organisms and P. tricornutum is considered the model organism for diatom biology and physiology, as well as for development of biotechnological approaches. A large number of molecular tools are available for P. tricornutum, like genetic transformation and genomic engineering based on nucleases such as TALEN and CRISPR/Cas9. Gene knock out or knock down will be performed to produce mutants partially or totally deprived of one or more genes of interest. A lipidomic phenotypic characterisation will lead to the characterisation of the functions of the enzymes encoded by the aforementioned genes.

The candidate will be involved in the construction of expression vectors for genomic engineering and in the genetic transformation of such vectors into P. tricornutum via biolistics. The candidate will take care of the genetic as well as phenotypic screening of the mutant strains by means of a screening procedure recently established in the lab. Promising strains will be analysed for their lipid content by GC-MS and LC-MS/MS in order to characterise the functions of the genes.

A percentage of the time of the candidate will be devoted to a collaborative project with a private company, which aims at improving lipid production in different marine protist.

Required qualifications:

  • Diploma equivalent to Licence professionnelle, with special options on biotechnologies, microbiology, plant biology, cell culture, molecular biology and/or biochemistry

Required skills:

  • Data manipulation and reporting.
  • Molecular cloning.
  • Axenic algal or other microorganism cultivation.
  • Effective written and oral communication skills.
  • Ability to work independently and in a group.
  • Abilitiy to contribute to multiple projects.
  • Ability to learn new topics and think critically and creatively for problem solving.

 

18 to 24 months.

Location : LPCV Grenoble (http://www.lpcv.fr/)

Send CV + motivation letter to Alberto Amato (alberto.amato@cea.fr) and Eric Maréchal (eric.marechal@cea.fr) before February 15, 2020

 Laboratoire de Physiologie Cellulaire & Végétale

UMR 5168 CNRS-CEA-INRA-Univ. Grenoble Alpes / INRA UMR 1417

IRIG, CEA-Grenoble

17 rue des Martyrs, 38000 Grenoble, France

 http://www.lpcv.fr/en

+33 (0)4 38 78 49 85

 

 

 

 

Contact : envoyer CV et lettre de motivation à Alberto Amato (alberto.amato@cea.fr) et Eric Maréchal (eric.marechal@cea.fr) AVANT LE 15 FEVRIER 2020

Send CV + motivation letter to Alberto Amato (alberto.amato@cea.fr) and Eric Maréchal (eric.marechal@cea.fr) BEFORE FEBRUARY 15, 2020

25 février 2019 Post_doctoral Associate Position (2-5 years) in Prague, Institute of Physiology

Research on Lipid Metabolism

Postdoctoral associate position is available in the laboratory of Dr. Ondrej Kuda at the Institute of Physiology of the Czech Academy of Sciences (IPHYS) in Prague. IPHYS is a leading national institution in research on normal and pathological physiology. The mission of the Institute is to enhance fundamental knowledge of the processes involved in metabolic regulation and its impact on cardiovascular, neuronal and brain function.
We are looking for curious, imaginative, independent and highly-motivated applicants holding a PhD with experience in biochemistry/physiology/molecular biology. Skills in any of the following: bioinformatics/programing, whole animal physiology, LC-MS/MS are a plus. The institute is bilingual (Czech/English) and good communication skills and a collaborative attitude are important.
The focus of Dr. Kuda’s lab is the metabolism and regulatory actions of bioactive lipids, specifically as related to pathways of glucose and lipid metabolism. We are interested in products of de novo lipogenesis such as branched fatty acid esters of hydroxy fatty acids (FAHFA), in anti-inflammatory & pro-resolving eicosanoids and docosanoids, and in endocannabinoid-like metabolites derived from omega-3 polyunsaturated fatty acids. We combine cutting edge biochemical, bioinformatics and LC-MS-based omic approaches to elucidate pathway regulation and relevance to the mechanisms underlying metabolic diseases such as type 2 diabetes and obesity. For additional information see lab website: http://www.fgu.cas.cz/en/departments/metabolism-of-bioactive-lipids
The position is initially funded for 2 years with possibility of extension for up to 5 years. Starting date can be anytime in the first half of 2019. Dr. Kuda’s lab is well funded by national research grants and institutional support from the Czech Academy of Sciences. The position salary is competitive and exceeds average compensation for similar positions in the local metropolitan area.
Applications should be sent directly to Dr. Kuda at ondrej.kuda@fgu.cas.cz before April 12th, 2019. Applications will not be reviewed until all below items are received. Applicants will be contacted to schedule an interview till April 12th.
1. Curriculum vitae
2. Two publications (attach PDFs). First author or with major contribution.
3. Cover letter: State why you qualify for the position and include description of relevant skills and experience
4. Copy of official diploma and any other academic records you wish to provide in support of your application
5. List of two to five references (with address and contact email) and indicate how these individuals are professionally related to you.
https://www.nature.com/naturecareers/job?id=676609

25 février 2019 Post-doctoral position in Lipidomics

A postdoctoral researcher fellowship position (18 months) in lipidomics/sterolomics is available at ISA (ANABIO-MS team)

Post-doctoral position in Lipidomics
A postdoctoral researcher fellowship position (18 months) in lipidomics/sterolomics is available at ISA (ANABIO-MS team) in close collaboration with Ecotoxicolgy group (Irstea). The fellowship funded by the ANR will be starting in September 2019.
Project
Aquatic ecosystems are exposed to environmental stressors such as chemical micropollutants from natural environment or anthropogenic activities. These chemical contaminations may result in alterations of the internal biochemical homeostasis of the aquatic organisms. The major limitation in the mechanistic knowledge of environmental chemical toxicity effects on aquatic organisms is the absence of molecular information notably at the genome wide scale in environmentally relevant species. The “omics” technologies – such as Transcriptomics, Proteomics, Lipidomics, Metabolomics – offer a great promises to help to elucidate molecular responses to exposures in aquatic organisms during specific and vulnerable life cycle stages. Lipid metabolism is the major fundamental metabolic pathway producing energy in animals. In fresh water organisms, lipids play a pivotal role in vulnerable stages like molting, reproduction, development. Recently, it has been shown that pharmaceutical drugs like hypolipidemic drugs have been detected in sewages, affecting the reproduction and population growth of crustacean amphipods (genus Gammarus). To understand and predict the effects of toxic exposures, it is crucial to describe the lipidome and to identify the affected metabolic networks. The project aim to gain a mechanistic understanding of toxic effects of hypolidiaemia drugs (statins and fibrates) on the sentinel organism, G. fossarum.
An innovative multi-omics approach (proteomics and lipidomics) will be developed to monitor metabolic perturbation on different development stages and reproductive cycles of G. fossarum after exposure with hypolidiaemia drugs.
For further details of the project, please follow the link : http://www.agence-nationale-recherche.fr/Project-ANR-18-CE34-0008
Profile
We seek a highly motivated and enthusiastic candidate with a PhD in the area of lipidomics with a focus on sterols analysis, or analytical chemistry or ecotoxicology with an interest in analytical chemistry.
The successful applicant will work at the Institute of Analytical Sciences (ANABIO-MS team) in collaboration with Ecotoxicology group at Irstea.
The requested skills include:
 Strong chemical background with a PhD in Analytical Chemistry or Biochemistry with hands-on mass spectrometry
 A significant level of technical knowledge and experience in mass spectrometry (Triple quadrupole, Q-TOF) including its applications in lipidomics or metabolomics
 Experience in biological sample preparation techniques
 Experience in chemometrics (intermediate to strong skills) and data mining will be an asset (R, Matlab).
 Evidence of peer-reviewed publications
 Good collaboration and communication skills (written and oral English)
Salary and appointments terms
The salary and appointment terms are consistent with the current rules for post-doctoral fellows. The period of employment is 18-months starting in September 2019.
Application
The successful candidate is expected to start in September 2019. The closing date for applications is 15th May 2019. Applications must be submitted as a single PDF containing:
 Current curriculum vitae including the list of publications
 A cover letter
 PhD diploma
 References (with letters of support)
Further information / contact
Sophie Ayciriex, PhD
Institute of Analytical Sciences (ISA),
CNRS UMR 5280 – ENS Lyon – University Claude Bernard Lyon 1
5 Rue de la Doua- 69100 Villeurbanne (France)
sophie.ayciriex@univ-lyon1.fr

25 février 2019 PhD student position

PhD student position on Microalgal Carbon and Lipid Metabolism

Available PhD student position on Microalgal Carbon and Lipid Metabolism

February 19, 2019

Topic: Manipulating redox balance to increase carbon reserve formation in microalgae

Host lab: The lab is located at Institute of Bioscience and Biotechnology of Aix Marseille (http://biam.cea.fr/drf/biam/Pages/accueil.aspx), which is part of The French Atomic and Alternative Energy Commission (CEA), and part of the French National Center for Scientific Research (CNRS), and University of Aix Marseille in the region Provence in the South of France.

Starting date: fall 2019 (3 years)

Funding: This project is funded by the CEA. The PhD student will receive a very competitive stipends plus all the social benefit.

Director of Thesis: Dr. Yonghua Li-Beisson

E-mail: yonghua.li@cea.fr

Scientific background and project: Microalgae requires only sun light and water to fix atmosphere CO2 into organic carbon. They therefore represent a promising platform not only for production of a range of biomaterials ranging from food, fuel to green chemistry application, but also are key to achieving a carbon neutral economy. Microalgae naturally have the capacity to produce starch and lipids; starch are precursors to bioethanol and lipids are precursors to biodiesel. But high amount of starch or lipid is made mostly under conditions when cell division and growth are compromised. Our team has recently discovered for the first time that redox communication occur between peroxisome and chloroplast, two of the three most energetic subcellular organelles, and we further shown that this redox communication plays an important role in regulation of photosynthesis, lipid and starch metabolism (Kong et al 2018 Plant Cell). In this PhD thesis, we aim to further explore the impact of altering redox state on carbon reserve formation in microalgae.

Profile desired: The candidate is expected to have already some experience working with molecular biology of Chlamydomonas. Experience in Confocal microscopy a plus. He/she will be trained in algal physiology, lipidomics and metabolism. He/she will work in close collaboration with other researchers, technicians and students of the group.

Reference: Kong F, Burlacot A, Liang Y, Legeret B, Alseekh S, Brotman Y, Fernie AR, Krieger-Liszkay A, Beisson F, Peltier G, Li-Beisson Y (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE 2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell

 

For more ref on related work : please visit the https://orcid.org/0000-0003-1064-1816

 

 

 

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